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Indeed, Ku70/80 has been proposed to perform at least two functions at telomeres. First, it protects telomeres ends from degradation, and second it regulates telomere length, possibly through a functional interaction with the telomerase holoenzyme [5, 6]. However, how Ku70/80 operates at telomere seems to differ among species. Ku70/80 associates with telomeric DNA ( 13) and binds to TRF1 and TRF2 ( 14, 15). Moreover, Ku has been reported to associate with hTERT and telomerase activity ( 16).

Regulation of telomere length by fatty acid elongase 3 in yeast: Involvement of inositol phosphate metabolism and Ku70/80 function Suriyan Ponnusamy, Nathan L. Alderson, Hiroko Hama, Jacek Bielawski, James C. Jiang, Rashna Bhandari, Solomon H. Snyder , S. Michal Jazwinski, Besim Ogretmen The Ku70/80 heterodimer is a key-player in the NHEJ pathway and binds to DNA termini with high affinity, where it helps to protect DNA ends from degradation and to recruit other NHEJ factors required for repair. The mechanism of Ku70/80 detachment from the DNA helix after completion of DNA repair is incompletely understood. The genetic disease Fanconi anemia (FA) results from mutations in a series of genes involved in a DNA repair pathway that helps process the damage caused by erroneous chemical cross-links between the two strands of the DNA double helix. The double-stranded breaks in DNA that arise from such cross-links can be repaired in an error-free manner or through an error-prone repair pathway. Pace et al The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. Here, we describe intramolecular interactions of the Ku70 C‐terminal domain, known as the SAP domain.

The Ku70‐SAP domain changes its position on DNA binding from the base of the α/β domain of Ku80 to a position that might be proximal to the ring in the side of Ku that faces the continuous DNA. The heterodimer Ku70/80 plays a critical role in DNA repair and cell death induction after damage. The aberrant expression and localization of these proteins fail to control DNA repair and apoptosis. sClusterin is the Ku70 partner that sterically inhibits Bax-dependent cell death after damage in some pathologic conditions.

Nr 3 • 2016 - Svensk Urologisk Förening - doczz

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Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system . Ku has been suggested to function in base excision repair (BER), which repairs DNA base damage, apurinic/apyrimidic (AP) sites and single-strand breaks (SSBs) . Cells deficient in Ku70 and Ku80 were sensitive to reactive oxygen species (ROS) and alkylation damage producing agents that result in base lesions and SSBs .

The data from Bennett et al. is consistent with the crystal structure as determined by Walker 2015-01-01 KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation Simplified, Ku70/80 complex is a sensor of DSBs that initiates the assembly of C-NHEJ complex to restore integrity of a DNA molecule. In fact, the role of Ku70/80 proteins is highly complex and integrates different aspects of DNA repair. 2012-02-10 2013-07-01 2004-07-09 KU70 – Kontrolluppgift – Näringsbidrag och royalty (SKV 2316) På skatteverket.se använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.
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Tested in Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse, Non-human primate, Rat, Xenopus laevis samples.

Se hela listan på academic.oup.com 2007-01-23 · Function; Names & Taxonomy Ku80 and XRCC6/Ku70. KYSGRDSLIF LVDASKAMFE 60 70 80 90 100 SQSEDELTPF DMSIQCIQSV YISKIISSDR DLLAVVFYGT EKDKNSVNFK 110 1 Definition. Ku70/Ku80 ist ein heterodimerer Proteinkomplex. Ku70/Ku80 bindet an freie DNA-Enden und ist in die Reparatur von Doppelstrangbrüchen durch die nicht-homologe Endverknüpfung involviert.
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2013-05-01 · When both Ku70/80 and RECQ1 were incubated with fork duplex in the presence of ATPγS, we observed a super-shifted species that is distinct from those obtained with binding of the fork DNA with Ku70/80 or RECQ1 alone (Fig. 3A, lane 3), indicating that Ku70/80 and RECQ1 can together bind the fork DNA structure. The Ku70/80 heterodimer rapidly responds to DSBs, stimulating recruitment of downstream NHEJ factors to protect, process, and ligate broken DNA ends.

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Sv. Urologi nr 1 2017 - Svensk Urologisk Förening

KU70 – Kontrolluppgift – Näringsbidrag och royalty (SKV 2316) På skatteverket.se använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor. The nuclear DNA repair protein Ku70/80 is a tumor-associated antigen displaying rapid receptor mediated endocytosis. / Fransson, Johan; Borrebaeck, Carl.. In: International Journal of Cancer, Vol. 119, No. 10, 2006, p.